goat anti human cd163 polyclonal antibody (R&D Systems)
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Goat Anti Human Cd163 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+polyclonal+anti+human+cd163/Human+Cystatin+A+Antibody/pmc11668767-51-1-6
Average 92 stars, based on 3 article reviews
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1) Product Images from "Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection"
Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2024.1505900
Figure Legend Snippet: Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
Techniques Used: Expressing, Isolation, Flow Cytometry
Figure Legend Snippet: Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.
Techniques Used: Expressing, Infection, Isolation, Staining, Flow Cytometry
Figure Legend Snippet: Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.
Techniques Used: Cell Culture, Isolation, Expressing, Flow Cytometry, Infection
Figure Legend Snippet: Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.
Techniques Used: Infection, Cell Culture, Incubation, Staining, Flow Cytometry
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