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goat anti human cd163 polyclonal antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat anti human cd163 polyclonal antibody
    Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and <t>CD163</t> proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
    Goat Anti Human Cd163 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+human+cd163/Human+Cystatin+A+Antibody/pmc11668767-51-1-6
    Average 92 stars, based on 3 article reviews
    goat anti human cd163 polyclonal antibody - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection"

    Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1505900

    Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
    Figure Legend Snippet: Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Techniques Used: Expressing, Isolation, Flow Cytometry

    Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.
    Figure Legend Snippet: Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.

    Techniques Used: Expressing, Infection, Isolation, Staining, Flow Cytometry

    Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.
    Figure Legend Snippet: Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.

    Techniques Used: Cell Culture, Isolation, Expressing, Flow Cytometry, Infection

    Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.
    Figure Legend Snippet: Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.

    Techniques Used: Infection, Cell Culture, Incubation, Staining, Flow Cytometry

    Related Articles

    Immunohistochemistry:

    Article Title: Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys
    Article Snippet: .. Antibodies used for IHC included rabbit monoclonal anti-human CD3 (SP7; Neomarkers), mouse anti-human Fascin (FCN01; Neomarkers), mouse anti-human CD68 (KP1; Dako), mouse anti-human macrophage (MAC387; Abcam), goat polyclonal anti-human CD163 (R&D Systems), mouse anti-human CD11c (5D11; Novocastra Laboratories), and mouse anti-human CD83 (1H4b; Novocastra Laboratories). ..

    Article Title: Availability of activated CD4 + T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys
    Article Snippet: .. Antibodies used for IHC included rabbit monoclonal anti-human CD3 (SP7; Neomarkers), mouse anti-human Fascin (FCN01; Neomarkers), mouse anti-human CD68 (KP1; Dako), mouse anti-human macrophage (MAC387; Abcam), goat polyclonal anti-human CD163 (R&D Systems), mouse anti-human CD11c (5D11; Novocastra Laboratories), and mouse anti-human CD83 (1H4b; Novocastra Laboratories). ..



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    Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and <t>CD163</t> proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
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    Image Search Results


    Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

    doi: 10.3389/fmicb.2024.1505900

    Figure Lengend Snippet: Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

    Techniques: Expressing, Isolation, Flow Cytometry

    Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.

    Journal: Frontiers in Microbiology

    Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

    doi: 10.3389/fmicb.2024.1505900

    Figure Lengend Snippet: Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.

    Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

    Techniques: Expressing, Infection, Isolation, Staining, Flow Cytometry

    Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.

    Journal: Frontiers in Microbiology

    Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

    doi: 10.3389/fmicb.2024.1505900

    Figure Lengend Snippet: Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.

    Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

    Techniques: Cell Culture, Isolation, Expressing, Flow Cytometry, Infection

    Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.

    Journal: Frontiers in Microbiology

    Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

    doi: 10.3389/fmicb.2024.1505900

    Figure Lengend Snippet: Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.

    Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

    Techniques: Infection, Cell Culture, Incubation, Staining, Flow Cytometry

    Figure 6. Tracing placental macrophage polarization trajectories identifies depletion of anti-inflammatory M2 macrophages and histiocytic intervillositis in highly positive SARS-CoV-2 placentae (A) Schematic of macrophage polarization from naive monocytes (M0) to pro-inflammatory M1 and anti-inflammatory M2. (B) Canonical markers for each subpopulation. #Caveats exist including potential differences by gestational age and between single-cell RNA and protein levels. (C) The 3,180 placental macrophages were analyzed by Monocle3, revealing pseudotime trajectories starting at M0 monocytes and trajectories going to M1 or M2 polarized subpopulations. (D) Using the pseudotime trajectory results, subpopulations were annotated based on predicted polarization states including intermediates (e.g., M0 to M1 is M0.M1). (E) Proportions of macrophages according to predicted polarization states. Error bars represent the standard error of the mean. (F and G) IHC staining for CD163, a classical macrophage marker. Images were taken at 403 original magnification. (G) Proportions of all spatial transcriptomes (see Figures 2 and S2C) were separated based on virus detection grouping from Figure 2 and the cluster analysis of SARS-CoV-2+

    Journal: Med (New York, N.Y.)

    Article Title: SARS-CoV-2 niches in human placenta revealed by spatial transcriptomics.

    doi: 10.1016/j.medj.2023.06.003

    Figure Lengend Snippet: Figure 6. Tracing placental macrophage polarization trajectories identifies depletion of anti-inflammatory M2 macrophages and histiocytic intervillositis in highly positive SARS-CoV-2 placentae (A) Schematic of macrophage polarization from naive monocytes (M0) to pro-inflammatory M1 and anti-inflammatory M2. (B) Canonical markers for each subpopulation. #Caveats exist including potential differences by gestational age and between single-cell RNA and protein levels. (C) The 3,180 placental macrophages were analyzed by Monocle3, revealing pseudotime trajectories starting at M0 monocytes and trajectories going to M1 or M2 polarized subpopulations. (D) Using the pseudotime trajectory results, subpopulations were annotated based on predicted polarization states including intermediates (e.g., M0 to M1 is M0.M1). (E) Proportions of macrophages according to predicted polarization states. Error bars represent the standard error of the mean. (F and G) IHC staining for CD163, a classical macrophage marker. Images were taken at 403 original magnification. (G) Proportions of all spatial transcriptomes (see Figures 2 and S2C) were separated based on virus detection grouping from Figure 2 and the cluster analysis of SARS-CoV-2+

    Article Snippet: FF-OCT cryosections and FFPE sections were fixed in pre-chilled 10% formalin for an hour at 4 C and blocked with appropriate blocking serum before staining for 30 min with monoclonal mouse-anti-SARS-CoV-2 spike antibody (GeneTex, Cat. GTX632604) diluted 1:500, polyclonal rabbit-anti-SARS-CoV-2 nucleocapsid antibody (GeneTex, Cat. GTX135357) diluted 1:200, and polyclonal goat-anti-human-CD163 antibody (R&D Systems, Cat. AF1607) diluted to 1 mg/mL.

    Techniques: Immunohistochemistry, Marker, Virus

    Detection of PRRSV receptors in SJPL cell line by immunofluorescence. The IFA was done using specific antibodies directed against CD151, CD163 and Sialoadhesin proteins in SJPL, PAM and MARC-145 cells. Negative control was obtained without primary antibody exposition. As an example of results, the negative controls illustrated are the FITC conjugated anti-mouse. All cell nuclei were stained with DAPI. Cells were visualized under UV exposition. Scale bar = 100 μm.

    Journal: Virology Journal

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

    doi: 10.1186/1743-422X-9-267

    Figure Lengend Snippet: Detection of PRRSV receptors in SJPL cell line by immunofluorescence. The IFA was done using specific antibodies directed against CD151, CD163 and Sialoadhesin proteins in SJPL, PAM and MARC-145 cells. Negative control was obtained without primary antibody exposition. As an example of results, the negative controls illustrated are the FITC conjugated anti-mouse. All cell nuclei were stained with DAPI. Cells were visualized under UV exposition. Scale bar = 100 μm.

    Article Snippet: The antibodies used were: rabbit polyclonal anti-human CD151 (Santa Cruz Biotechnology, CA, USA); mouse monoclonal anti-pig CD163 (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human CD163 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells; mouse monoclonal anti-pig CD169 (synonym: Sialoadhesin, Siglec-1) (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human Siglec-1 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells.

    Techniques: Immunofluorescence, Negative Control, Staining

    Infectious viral particles production in PRRSV-infected SJPL cells following five consecutive passages. PRRSV IAF-Klop strain was passaged serially in MARC-145 and SJPL cells as described in the materials and methods section. The amount of the infectious viral particles recovered after each passage was determined in MARC-145 cells. The virus titers were expressed as TCID 50 per 10 6 cells. The initial viral inoculum (inocul) used to infect both cell lines was 10 3.3 TCID 50 /10 6 cells.

    Journal: Virology Journal

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

    doi: 10.1186/1743-422X-9-267

    Figure Lengend Snippet: Infectious viral particles production in PRRSV-infected SJPL cells following five consecutive passages. PRRSV IAF-Klop strain was passaged serially in MARC-145 and SJPL cells as described in the materials and methods section. The amount of the infectious viral particles recovered after each passage was determined in MARC-145 cells. The virus titers were expressed as TCID 50 per 10 6 cells. The initial viral inoculum (inocul) used to infect both cell lines was 10 3.3 TCID 50 /10 6 cells.

    Article Snippet: The antibodies used were: rabbit polyclonal anti-human CD151 (Santa Cruz Biotechnology, CA, USA); mouse monoclonal anti-pig CD163 (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human CD163 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells; mouse monoclonal anti-pig CD169 (synonym: Sialoadhesin, Siglec-1) (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human Siglec-1 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells.

    Techniques: Infection

    PRRSV replication kinetics in SJPL cells. MARC-145 and SJPL cells were infected at 1 MOI with PRRSV IAF-Klop ( A , B ) and LV ( C , D ) strains. At different time pi, the infectious viruses recovered from the cell culture medium ( B , D : supernatant fraction) and the cells ( A , C : cell fraction) were titered in MARC-145 cells. Experiment was done in triplicate.

    Journal: Virology Journal

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

    doi: 10.1186/1743-422X-9-267

    Figure Lengend Snippet: PRRSV replication kinetics in SJPL cells. MARC-145 and SJPL cells were infected at 1 MOI with PRRSV IAF-Klop ( A , B ) and LV ( C , D ) strains. At different time pi, the infectious viruses recovered from the cell culture medium ( B , D : supernatant fraction) and the cells ( A , C : cell fraction) were titered in MARC-145 cells. Experiment was done in triplicate.

    Article Snippet: The antibodies used were: rabbit polyclonal anti-human CD151 (Santa Cruz Biotechnology, CA, USA); mouse monoclonal anti-pig CD163 (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human CD163 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells; mouse monoclonal anti-pig CD169 (synonym: Sialoadhesin, Siglec-1) (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human Siglec-1 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells.

    Techniques: Infection, Cell Culture

    Procaspases 3/7 activation in SJPL cells infected by PRRSV. MARC-145 and SJPL cells were infected at 0.5 MOI with PRRSV IAF-Klop strain or incubated with a combination of four apoptotic inducers (actinomycin D, vinblastine sulfate, cycloheximide and puromycin) as a positive control. At 24 hrs post-incubation with the apoptotic inducers, MARC-145 cells have developed high CPE level compared to SJPL cells which showed low to mild CPE. At 72 hrs, PRRSV infected cells were disrupted for the detection of caspase 3 using a specific fluorogenic substrate. The results were expressed as relative fluorescence released (relative fluorescence units or RFU) per second per μg of cell lysates. Values are presented as ± standard deviation (SD). When 2 sets of data within a cell type are labeled with superscripts of different letters or when only one set is labeled with a superscript, it indicates that these 2 sets of data are statistically different ( P < 0.05). When 2 sets of data from different cell types are labeled with asterisk, it indicates that these 2 sets of data are statistically different (*** P < 0.001).

    Journal: Virology Journal

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

    doi: 10.1186/1743-422X-9-267

    Figure Lengend Snippet: Procaspases 3/7 activation in SJPL cells infected by PRRSV. MARC-145 and SJPL cells were infected at 0.5 MOI with PRRSV IAF-Klop strain or incubated with a combination of four apoptotic inducers (actinomycin D, vinblastine sulfate, cycloheximide and puromycin) as a positive control. At 24 hrs post-incubation with the apoptotic inducers, MARC-145 cells have developed high CPE level compared to SJPL cells which showed low to mild CPE. At 72 hrs, PRRSV infected cells were disrupted for the detection of caspase 3 using a specific fluorogenic substrate. The results were expressed as relative fluorescence released (relative fluorescence units or RFU) per second per μg of cell lysates. Values are presented as ± standard deviation (SD). When 2 sets of data within a cell type are labeled with superscripts of different letters or when only one set is labeled with a superscript, it indicates that these 2 sets of data are statistically different ( P < 0.05). When 2 sets of data from different cell types are labeled with asterisk, it indicates that these 2 sets of data are statistically different (*** P < 0.001).

    Article Snippet: The antibodies used were: rabbit polyclonal anti-human CD151 (Santa Cruz Biotechnology, CA, USA); mouse monoclonal anti-pig CD163 (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human CD163 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells; mouse monoclonal anti-pig CD169 (synonym: Sialoadhesin, Siglec-1) (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human Siglec-1 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells.

    Techniques: Activation Assay, Infection, Incubation, Positive Control, Fluorescence, Standard Deviation, Labeling

    Relative expression of IFNα (A), IFNβ (B), IFNγ (C) and TNF-α (D) mRNA of SJPL and MARC-145 cells induced by PRRSV. MARC-145 and SJPL cells were infected at 0.5 MOI with PRRSV IAF-Klop strain or transfected with poly (I:C) as a positive control or treated with LPS as an IFNγ inducer. mRNA relative expression of IFNα (A), IFNβ (B), IFNγ (C) and TNF-α (D) was measure at 72 hrs by qRT-PCR in PRRSV infected or poly (I:C) treated cells. Values are presented as ± standard deviation (SD). When 2 sets of data within a cell type are labeled with superscripts of different letters or when only one set is labeled with a superscript, it indicates that these 2 sets of data are statistically different ( P < 0.05). When 2 sets of data from different cell types are labeled with asterisk, it indicates that these 2 sets of data are statistically different (* P < 0.05, ** P < 0.01, *** P < 0.001).

    Journal: Virology Journal

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

    doi: 10.1186/1743-422X-9-267

    Figure Lengend Snippet: Relative expression of IFNα (A), IFNβ (B), IFNγ (C) and TNF-α (D) mRNA of SJPL and MARC-145 cells induced by PRRSV. MARC-145 and SJPL cells were infected at 0.5 MOI with PRRSV IAF-Klop strain or transfected with poly (I:C) as a positive control or treated with LPS as an IFNγ inducer. mRNA relative expression of IFNα (A), IFNβ (B), IFNγ (C) and TNF-α (D) was measure at 72 hrs by qRT-PCR in PRRSV infected or poly (I:C) treated cells. Values are presented as ± standard deviation (SD). When 2 sets of data within a cell type are labeled with superscripts of different letters or when only one set is labeled with a superscript, it indicates that these 2 sets of data are statistically different ( P < 0.05). When 2 sets of data from different cell types are labeled with asterisk, it indicates that these 2 sets of data are statistically different (* P < 0.05, ** P < 0.01, *** P < 0.001).

    Article Snippet: The antibodies used were: rabbit polyclonal anti-human CD151 (Santa Cruz Biotechnology, CA, USA); mouse monoclonal anti-pig CD163 (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human CD163 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells; mouse monoclonal anti-pig CD169 (synonym: Sialoadhesin, Siglec-1) (AbD Serotec, Oxford, United Kingdom) for PAMs cells or goat polyclonal anti-human Siglec-1 (Santa Cruz Biotechnology, CA, USA) for MARC-145 and SJPL cells.

    Techniques: Expressing, Infection, Transfection, Positive Control, Quantitative RT-PCR, Standard Deviation, Labeling

    A mouse anti-human FABP1 monoclonal antibody (1∶100 dilution), goat anti-human CD163 polyclonal antibodies (1∶100 dilution), goat anti-human MBP polyclonal antibodies (1∶100 dilution), and a mouse anti-human alpha smooth muscle actin monoclonal antibody (1∶50, clone 1A4, DAKO, Glostrup, Denmark) were used. Magnification: 400×.

    Journal: PLoS ONE

    Article Title: Fatty Acid Binding Protein 1 Is Related with Development of Aspirin-Exacerbated Respiratory Disease

    doi: 10.1371/journal.pone.0022711

    Figure Lengend Snippet: A mouse anti-human FABP1 monoclonal antibody (1∶100 dilution), goat anti-human CD163 polyclonal antibodies (1∶100 dilution), goat anti-human MBP polyclonal antibodies (1∶100 dilution), and a mouse anti-human alpha smooth muscle actin monoclonal antibody (1∶50, clone 1A4, DAKO, Glostrup, Denmark) were used. Magnification: 400×.

    Article Snippet: For the immunohistochemical analysis of FABP1, nasal polyp tissue on the slides was treated with 0.3% H 2 O 2 for 20 min to block endogenous peroxidase and then incubated at 4°C overnight with a mouse anti-human FABP1 monoclonal antibody (1∶100 dilution; Abcam), goat anti-human CD163 polyclonal antibodies (1∶100 dilution; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), goat anti-human MBP polyclonal antibodies (1∶100 dilution; Santa Cruz Biotechnology Inc.), and a mouse anti-human alpha smooth muscle actin monoclonal antibody (1∶50, clone 1A4, DAKO).

    Techniques: